A diagnostic method and kits for detecting sub-acute ruminal acidosis in livestock animals. The method comprises collecting a blood sample from a livestock animal, contacting the blood sample with a kit provided with at least one marker derived from at least one acute phase protein associated with inflammatory responses. The marker is an antibody configured to react with said acute phase protein. The antibody reacts with the acute phase protein present in the blood sample as a consequence of an inflammatory response to sub-acute ruminal acidosis thereby providing a detectable and preferably, a quantifiable result. The marker comprises at least one antibody configured to react with serum amyloid-A or haptoglobin. The diagnostic kit comprises a solid support structure provided with at least one marker configured to react with an acute phase protein. The diagnostic kit may be an ELISA kit Alternatively, the diagnostic kit may be a paper strip.
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