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Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby
Directed evolution methods for improving polypeptide folding and solubility and superfolder fluorescent proteins generated thereby
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机译:用于改善多肽折叠和溶解性的定向进化方法以及由此产生的超文件夹荧光蛋白
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摘要
The current invention provides methods of improving folding of polypeptides using a poorly folding domain as a component of a fusion protein comprising the poorly folding domain and a polypeptide of interest to be improved. The invention also provides novel green fluorescent proteins (GFPs) and red fluorescent proteins that have enhanced folding properties.
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