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Mono-specific polyclonal antibodies and methods for detecting Clostridium difficile Toxin A
Mono-specific polyclonal antibodies and methods for detecting Clostridium difficile Toxin A
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机译:单特异性多克隆抗体和艰难梭菌毒素A的检测方法
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摘要
A method is provided for the purification of Clostridium difficile Toxin A antigen comprising reacting impure Toxin A with immobilized mono-specific polyclonal antibodies. The polyclonal antibodies are coupled to a hydrazide group containing matrix such as hydrazide activated agarose gel. The immobilized antibody is specific for Toxin A and will greatly purify Toxin A from a Toxin A containing solution. Antibodies raised to Toxin A purified according to the method are of higher activity than antibodies produced from prior art purified Toxin A.
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