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Method of performing reverse transcription reaction using reverse transcriptase encoded by non-LTR retrotransposable element
Method of performing reverse transcription reaction using reverse transcriptase encoded by non-LTR retrotransposable element
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机译:使用非LTR逆转座子元件编码的逆转录酶进行逆转录反应的方法
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摘要
The present invention relates to a method of preparing a cDNA molecule which includes: contacting an RNA molecule, in the presence of dNTPs, with a non-LTR retrotransposon protein or polypeptide having reverse transcriptase activity under conditions effective for production of a cDNA molecule complementary to the RNA molecule, the contacting being carried out in the absence of a target DNA molecule of the non-LTR retrotransposon protein or polypeptide; and isolating the cDNA molecule. The preferred non-LTR retrotransposon protein or polypeptide is an R2 protein or polypeptide.
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