Methods to improve the performance of a subtilisin in Systems of low and high concentration of detergent, a Detergent Composition and Method for producing

摘要

Method for improving performance of a system that in a low concentration of Detergent with less than 800 ppm of Detergent Components present in the wash water,Comprising: (a) replace a residue of amino acid in one or more positions in a subtilisin Precursor to produce a variant of subtilisin where the substitution Alters the load in that position for loading more negative or less positive compared with the precursor.(b) variant and rehearse in a system of low concentration of Detergent with less than 800 ppm of Detergent Components present in the wash water by comparing the ability of the precursor and the option to remove a stain.Method for improving performance of a subtilisin in high concentration system with 2000 ppm of Detergent components of Detergent present in the wash water,Comprising: (a) replace a residue of amino acid in one or more positions in a subtilisin Precursor to produce a variant of subtilisin where the substitution Alters the load in that position for loading more positive or less negative compared with the precursor.(b) variant and rehearse in a high concentration of Detergent with 2000 ppm of Detergent Components present in the wash water by comparing the ability of the precursor and the option to remove a stain. Method for producing Detergent composition.