The process of semipurificau00e7u00e3o and extraction of plasma proteu00ecnas sangu00fcu00ecneo human and animal and kit for protein molecular weight marker, based on two-phase system employing surfactant

摘要

The process of semipurificau00e7u00e3o and extraction of proteins from human and animal blood plasma, and kit for protein molecular weight marker, based on the system of the present invention two employing surfactant is process of semipurificau00e7u00e3o proteins, preferably.Human blood plasma and a kit for maecador molecular weight protein using a system of phase separation on the basis of the non-ionic surfactant in the presence of electrolyte.More specifically, the present invention consists of a process of semipurificau00e7u00e3o and extraction of proteins from human blood plasma and in the production of a kit for protein molecular weight marker based on micellar extraction, with the employment of the surfactant.Although the system is not able to focus on a single protein fraction in the phase rich in surfactant protein, some fractions have a preference in migrate to the upper phase, while others remain in the lower phase. So, we have a system of protein separation, since we split a complex system into two major protein fractions.The kit is to drastically reduce the cost in the production of the marker, since the proteins extracted or migrated to the phase rich in surfactant, once identified, can be used as a reference to estimate the molecular weight.