A method for producing recombinant viruses containing nucleotide sequences encoding all or part of heterologous proteins, DNA constructs, resulting recombinant viruses and vaccine compositions containing such recombinant viruses to immunize against flaviviruses and / or other pathogens.

摘要

METHOD FOR THE PRODUCTION OF RECOMBINING VIRUSES AND RECOMMINATING VACCINUS VIRUSES AND INVOLVING COMPOSITIONS OF THE ALL OR PART OF HETEROLOGICAL PROTEINS, DNA CONSTRUCTS recombinant virus production by cloning and expressing nucleotide sequences coding for all or part of heterologous proteins by the following method: (a) modification of heterologous nucleotide sequences so that they, when cloned and expressed in the vector virus, have, at its 5 'portion, nucleotides present at the 5' end of the NS1 gene of this vector virus or other functionally equivalent viruses or sequences, and at its 3 'portion, the genomic region corresponding to all or part of the protein's stem and anchor domains And this vector virus or other viruses or sequences functionally equivalent, and thus not compromising the structure and replication of said vector virus; (b) insertion of the modified heterologous sequences in (a) into the intergenic region at the level of structural protein E and non-structural vector virus NS1; c) Obtaining non-pathogenic recombinant virus and possessing immunological properties, containing the sequences stably integrated into the viral genome as inserted into the region described in (b) and thus expressing the heterologous antigen so that it induces an appropriate immune response. Additionally, vaccine compositions are also provided to immunize against Flavivirus and / or other pathogens, consisting essentially of the virus obtained according to the above method.