recombinant sucrose synthase production method, its use in the manufacture of sucrose determination kits, adp glucose production and production of transgenic plants whose leaves and storage organs accumulate high adp and starch glucose contents

摘要

METHOD OF PRODUCING RECOMBINATING SACAROSIS SYNTASE, USE OF THE SAME IN THE MANUFACTURE OF KITS FOR DETERMINING SACAROSIS, ADP GLUCOSE PRODUCTION AND PRODUCTION OF TRANSGENCIES PLANTS WHICH LAMPS AND DAMAGES OF DAMAGED ALAMAGERS The present invention relates to a recombinant sucrose synthase production medium, or its use in the manufacture of kits for the determination of sucrose, production of ADPglucose and production of transgenic plants whose leaves and storage organs accumulate high levels of ADPglucose and starch. One method is described for the efficient production of large amounts of actively soluble recombinant SS by expression of the gene encoding SS gene in an Escherichia coli strain. The expression vector used means that the recombinant SS produced has a histidine end that facilitates rapid purification. In addition, he describes sequences of mutated versions of the SS gene encoding SS isoforms suitable for ADPG production. Using the "wild type" and "mutated" versions of recombinant SS, an efficient method is described for the production of ADPG and UDPG. It also describes the use of SS for the production of sucrose assay kits. Finally, he describes the production and production of transgenic plants that overexpress the SS gene gene, either constitutively or in storage sheets or organs, and which have a high content (both in leaves and storage tissues) of sucrose, ADPG, G6P and starch as a result of SS ADPG synthesizing activity.