Method of culturing human mezenchymal stem cells, particularly for facilitating fracture healing process, and bioreactor for carrying out the method

摘要

the essence of a solution is in the way pestovu00e1nu00ed mezenchymovu00fdch stem research.in which asepticku00e9m department mononuclear research of drenovu00e9 blood are those used in the low hustote into sterile plastic kultivacnu00edch jars and pestovu00e1ny for approximately one to three weeks in mu00e9diu cellgro.sup.tm.n.hematopoietic stem cell, approved for clinical pouzitu00ed with pru00eddavkem 10% human serum and suplementu, while allowing during cultivation are at one pridu00e1ny supplements.without need odmu00fdvat neadherentnu00ed haematopoietic cellswithout need change zivnu00fd solution and without any need to intervene in a 100 foot kultivacnu00edho system for beznu00fdch conditions for pestovu00e1nu00ed tku00e1novu00fdch cultures.for pestovu00e1nu00ed mezenchymovu00fdch stem research in uzavrenu00e9m kultivacnu00edm system for clinical pouzitu00ed of rekonstrukcnu00edortopedie is proposed a simple, uh, bioreactor,consisting of kazetove razenu00fdch kultivacnu00edch jars with filters for security sterile vu00fdmeny air and sasepticku00fdmi inputs for deploying and harvesting research and for doplnovu00e1nu00ed suplementu,spocu00edvaju00edcu00edch volneve suitable nosici.