USE OF 13C-NMR TO DETECT BINDING

摘要

Methods of detecting binding of a putative ligand to a 13C-enriched target molecule, methods of screening for compounds which bind to a 13C-enriched target molecule, methods for calculating the dissociation constant of a ligand compound which binds to a 13C-enriched target molecule, and methods employed in the determination of the specific amino acids in a 13C-enriched target molecule affected by the binding of a ligand, as well as compounds identified by these screening methods, are provided herewith.