A NOVEL THERMOSTABLE PROTEIN CONJUGATE AND A PROCESS FOR THE CONJUGATION OF THERMOSTABLE AMYLASE WITH ANTIBODY

摘要

a) dissolving α-amylase in a buffer of neutral pH to obtain a solution and adding a cross-linking agent such as hereindescribed to the solution, b) incubating the solution for 2 - 24 hrs in a manner such as hereindescribed, c) optionally removing the excess cross-linking agent by dialysis in a buffer of pH 7 to 9.5 and adjusting the pH if desired, d) adding an antibody such as hereindescribed to the solution and incubating the same overnight, e) adding ammonia-donating compounds such as hereindescribed for saturation of unconjugated enzyme, f) changing the buffer and thereby obtaining α-amylase-antibody conjugate which is suspended in neutral pH and storing the amylase-antibody conjugate at4°Cto-20°C.