A SEMINESTED POLYMERASE CHAIN REACTION (PCR) METHOD FOR RAPID DETECTION AND SPECIFIC IDENTIFICATION OF MYCOBACTERIUM CHELONAE

摘要

: This invention relates to a seminested polymerase chain Reaction (PCR) method and oligonucleotides for rapid detection and specific identification of Mycobacterium chelonae (M.cheJonae ). The present invention relates to a new seminested Polymerase Chain Reaction (PCR) method and oligonucleotides for rapid and specific identification of rapid growers of mycobacteria -Mycobacterium chelonae species, using species-specific primers derived from the ITS sequences of mycobacteria. The ITS region is considered to be less prone to selective pressure and consequently is expected to have accumulated a higher percentage of mutatit than the corresponding ribosomal DNA.