A PROCESS FOR PREPARING A BIOCIDE USED FOR CONTROLLING BLACK ROT DISEASE IN TEA PLANT .

摘要

A process for preparing a novel biocide comprising a consortium of two bacterial and two fungal strains for control1ing a severe disease of tea plants caused by pathoganic fungal strains namely corticium invisum and corticium theae. The preparation of the biocide is made by mixing the four ingredients strains at the ratio of 4:3 : 2:1 and the cultures are prepared in the following manner: a) Transferring the Bacillus subti1 is and Pseudomanas putida cultures into mother culture memdium, b) allowing the mother cultures to grow for 3-7 days, c) transferring the grown mother cultures to main culture medium and a 1 lowing the main cultures to grow for 3-7 clays. d) transferring the Trichoderma viridi and Trichoderma harzianum cultures in the mother culture memdiumi e) a1 lowing the mother cultures to grow for 15 days, f) transferring the mother cultures to the mam culture medium and allowing the main cultures to grow for 15 days. g) mixing the said ready cultures - Bacillus subti1is Pseudomonas putida, Trichoderma viridi and Trichoderma harzianum in a ratio of 4:3:2:1 to obtain the biocide.