A method for stimulating the teats of the udder of an animal, pulsator, and method for controlling of the milking process by means of the pulsation vacuum.

• 1. 清洗奶品动物挤奶器单元的挤奶器单元反冲和乳头浸液系统 [P] . 中国专利： CN104285821B . 2018.03.16
• 2. 清洗奶品动物挤奶器单元的挤奶器单元反冲和乳头浸液系统 [P] . 中国专利： CN104285821A . 2015-01-21
• 3. A METHOD OF STIMULATING THE TEATS OF THE UDDER OF AN ANIMAL, A PULSATOR, AND A METHOD OF CHECKING THE MILKING PROCESS ON THE BASIS OF THE PULSATION VACUUM [P] . 欧洲知识产权局专利： EP1951032A1 . 2008-08-06

  机译： 一种刺激动物乳味的方法，一种脉动器，以及一种根据脉动真空度检查挤奶过程的方法

• 4. A METHOD OF STIMULATING THE TEATS OF THE UDDER OF AN ANIMAL, A PULSATOR, AND A METHOD OF CHECKING THE MILKING PROCESS ON THE BASIS OF THE PULSATION VACUUM [P] . 世界知识产权组织专利： WO2007057125A1 . 2007-05-24

  机译： 一种刺激动物乳味的方法，一种脉动器，以及一种根据脉动真空度检查挤奶过程的方法

• 5. METHOD OF DETERMINING STIMULATING EFFECT OF MILKING APPARATUS UPON ANIMAL UDDER TEAT RECEPTORS [P] . SU1020089A1 . 1983-05-30

  机译： 测定乳汁对动物乳腺感受器刺激效果的方法

• 1. Control strategy and methods for continuous direct compression processes [J] . Yasuhiro Suzuki, Hirokazu Sugiyama, Manabu Kano, 亚洲药物制剂科学(英文) . 2021,第002期
• 2. A Randomized Trial to Study the Effect of Automatic Cluster Remover Settings on Milking Performance, Teat Condition, and Udder Health [C] . Annual Meeting of The National Mastitis Council . 2020
• 3. Effects of gel teat cleaning and sanitizing on raw milk quality and udder health. [D] . Ingawa, Kasimu Hudu. 1990
• 4. The Effect of Post Milking Teat Dip and Suckling on Teat Skin Condition Bacterial Colonisation and Udder Health [O] . M. D. Rasmussen, H. D. Larsen 1998
• 5. Contagem bacteriana da superfície de tetas de vacas submetidas a diferentes processos de higienização, incluindo a ordenha manual com participação do bezerro para estimular a descida do leite Bacterial counts on the surface of the teats of cows milked under different methods of udder preparation, including cows milked by hand and stimulated by suckling a calf [O] . José Renaldi Feitosa Brito, Maria Aparecida Vasconcelos Paiva e Brito, Rui da Silva Verneque 2000
• 6. Military Standard: Sanitary Standards for Equipment and Methods for the Handling of Milk and Milk Products in Bulk Milk Dispensing Operations. [R] . 1982

• 1. Reducing Efficiencies of the Commonly Used Heat Treatment Methods and Fermentation Processes on Aflatoxin M1 in Naturally Contaminated Fresh Cow Milk [J] . G. K. Omeiza ,M. Mwanza ,S. I. Enem . 兽医学（英文） . 2018,第8期
• 2. BACKGROUND： Vibrio vulnifi cus inside the body could activate the NF-!B signaling pathwayand initiate the inflammatory cascade. The lung is one of the earliest organs affected by sepsisassociated with acute lung injury. High mobility group protein B1 （HMGB1） is an important late-actingpro-infl ammatory cytokine involving in the pathophysiology of sepsis. It is also involved in the injuryprocess in the lung, liver and intestine. There has been no report on the involvement of HMGB1 inVibrio vulnifi cus sepsis-induced lung injury.METHODS： Sixty rats were randomly divided into a normal control group （group A, n=10） anda Vibrio vulnificus sepsis group （group B, n=50）. Sepsis was induced in the rats by subcutaneousinjection of Vibrio vulnificus （concentration 6×108 cfu/mL, volume 0.1 mL/100g）） into the left lowerlimbs. The rats in group B were sacrifi ced separately 1, 6, 12, 24, and 48 hours after the infection.Their lungs were stored as specimens, lung water content was measured, and lung pathology wasobserved under a light microscope. The expressions of the HMGB1 gene and protein in the lungswere detected by RT-PCR and Western blot. Data were analyzed with one-way analysis of variance（ANOVA） and the LSD method for pair-wise comparison between the two groups. P〈0.05 wasconsidered statistically signifi cant.RESULTS： Compared to group A （0.652±0.177）, HMGB1 mRNA expression in the lungs ofgroup B was signifi cantly higher at 0 hour （1.161±0.358, P=0.013）, 24 hours （1.679±0.235, P=0.000）,and 48 hours （1.258±0.274, P=0.004） （P〈0.05）, and peaked at 24 hours. Compared to group A（0.594±0.190）, HMGB1 protein expression at 6 hours （1.408±0.567, P=0.026） after infection wassignificantly increased （P〈0. 05）, and peaked at 24 hours （2.415±1.064, P=0.000） after infection.Compared to group A （0.699±0.054）, lung water content was significantly increased at 6 hours（0.759±0.030, P=0.001）,12 hours （0.767±0.023, P=0.000）, 24 hours （0.771±0.043, P=0.000） and 48hours （0.789±0.137, P=0.000） after infection （P〈0.05）. Compared to group A, pathological changesat 12 hours in group B indicate marked pulmonary vascular congestion, interstitial edema andinfl ammatory infi ltration. Alveolar cavity collapse and boundaries of the alveolar septum could not beclearly identifi ed.CONCLUSION： Vibrio vulnifi cus sepsis can lead to injury in rat lungs, and increased HMGB1expression in lung tissue may be one of the mechanisms for injury from Vibrio vulnifi cus sepsis. [J] . Qiao-meng Qiu ,Zhong-wang Li ,Lu-ming Tang . 世界急诊医学杂志(英文) . 2011,第4期
• 3. Moisture Control Methods in Silk Reeling Process of Tobacco Based on the Random Forest Regression [J] . Bo Ma . 统计学期刊（英文） . 2015,第5期
• 4. Milk Spoilage: Methods and Practices of Detecting Milk Quality [J] . Michael Lu ,Yvonne Shiau ,Jacklyn Wong . 食品与营养科学（英文） . 2013,第7期
• 5. 人乳头瘤病毒转基因动物模型:方法仍待改进 [C] . 左国伟 ,重庆医科大学实验动物中心 ,吕凤林 . 第五届国际转基因动物学术研讨会 . 2005
• 6. 基于流式细胞术和Toehold介导的链置换反应检测甲状腺乳头状癌相关的hsa-miR-146b-5p的新方法 [A] . 吴越 . 2021