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New expression system from Rhodococcus

机译:红球菌的新表达系统

摘要

An isolated polynucleotide comprising the kstD promoter from Rhodococcus erythropolis. The polynucleotide can very advantageously be used as a controllable transcription activator. Said controlling function can be provided by providing said isolated polynucleotide with a nucleotide sequence encoding a transcription regulator of said promoter. In the present invention, such a transcription regulator may be externally induced, such as by introduction of steroidal compounds. In an alternative embodiment of the present invention the isolated polynucleotide may comprise the kstR gene or a homologue or a functional part thereof as the transcription regulator of the kstD promoter.
机译:分离的多核苷酸,其包含来自红红球菌的kstD启动子。该多核苷酸可以非常有利地用作可控制的转录激活剂。可以通过向所述分离的多核苷酸提供编码所述启动子的转录调节子的核苷酸序列来提供所述控制功能。在本发明中,这种转录调节剂可以是外部诱导的,例如通过引入类固醇化合物。在本发明的另一个实施方案中,分离的多核苷酸可以包含kstR基因或其同源物或功能部分作为kstD启动子的转录调节子。

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