The process of micropropagau00c7u00c3o of Oliveira for in vitro culture of axillary buds

摘要

Using the micropropagau00c7u00c3o in vitro axillary buds was the parent at a low cost, cultivate de Oliveira "Galega vulgar", recalcitrant to classics of vegetative Multiplication Processes of species (grafting and woody cuttings and semi woody).The process consists in the culture conditions of asepsis of axillary buds from the branches of the year of adult plants using as substrate compounds by means of nutritional macro and micro nutrients, carbohydrates, vitamins and growth regulators, which are modified in funu00c7 Preparation of the development phase in the culture.1 - the installation; 2 - multiplication and elongation; 3 - rooting. The aclimatizau00c7u00c3o plants was carried out in greenhouse environment control. As mentioned above, the procedure has been tested in cultivating Portuguese "Galega vulgar"; however with slight modifications, be adapted to other recalcitrant cultivars.The amendments made to the technology currently available allow the obtaining of a high number of plants in a short period of time, and compatible with a commercial use costs.