METHOD FOR PREDICTION OF HYPERTENSIVE HEART DESEASE COURSE DESTABILIZATION

摘要

A method for predictions of hypertensive heart desease course destabilising comprises taking of 2 ml of patient blood, obtaining of 0,1 mls of Serum. The precipitation of blood proteins with the aid of 0.9 ml of 20 % solution of trichloroacetic acid (TCA) is carried out. An equal volume of 1 ml 0.1 M 2.4- DNPH (2.4- Dinitrophenylhydrazine) to denatured protein is added, which is dissolved in 2H HCl and ethanol and kept in boiling bath before complete dissolution of 2.4-DNPH. In control sample an equal volume is added 2H He, stored at a room temperature for 1 hour and then samples are centrifuged at 6000 g during 15-20 min. The precipitate is 3 times washed out by ethanole-ethyl acetate (1:1)solution, centrifuged at 6000 g for 15-20 min, for extraction of lipids and 2.4-DNPH, that did not react with carbonyl groups of oxidated proteins. Obtained precipitate is dried in order to remove ethanol-ethyl acetate solvent and is dissolved in 8 M solution of urea. Urea is added to a precipitate in volume of 4 ml and retained in boiling bath during 5 mines up to complete dissolution. Optical density of dinitrophenylhydrazones, which were formed, is registered on a spectrophotometer at wavelength of 370 nm. The result of phenylhydrazones determination is expressed in terms of extinction units on 1 ml of blood serum. And at the content of phenylhydrazones more than 5.5 D370/ ml one can conclude about destabilising of hypertensive heart desease course.