Process for obtaining endopolygalacturonase enzyme by culturing recombinant microbial strains overexpressing Kluyveromyces marxianus epg2 gene.

摘要

Process for obtaining endopolygalacturonase enzyme by culturing recombinant microbial strains overexpressing the gene EPG2 Kluyveromyces marxianus. cloning and sequencing EPG2 gene and construction of yeast strains that produce and secrete the enzyme in large amount endopolygalacturonase and very stably described. The enzyme produced can be used in depectinización fruit juice, vegetables, plant macerating and increased juice yield, animal feed formulation and the degradation of plant residues.