Fluorescent probes hybridizing with little background noise.

摘要

Pair of FRET hybridization probes consisting of a first oligonucleotide carrying a FRET donor entity and a second oligonucleotide carrying a FRET acceptor entity, wherein one oligonucleotide carrying the first unit FRET is labeled at its 3 'and the semi Gundo oligonucleotide fragment marked on its 5 '', and that said terminal fragments base pair with contiguous target nucleic acid fragments or fragments which are only separated by one or two radicals, so that the oligonucleotide carrying the donor fluorescent entity is carrying at least one second unit, which is capable of inhibiting nitroindole fluorescence emission from said donor fluorescent entity.