Preparation of serums for growth factor rich autological plasmas comprises controlled centrifugation of the supernatant layer of a centrifuged blood sample, for amicon based ultrafiltration

摘要

Preparing serum or autologous plasma rich in growth factors comprises removing blood from the patient in appropriate conditions to prevent lipemic sera applying an anticoagulant centrifuging blood centrifuging the supernatant extracting plasma closest to the interface with red blood cells and focus through a phase of ultrafiltration. Preparing serum or autologous plasma rich in growth factors comprises removing blood from the patient in appropriate conditions to prevent lipemic sera applying an anticoagulant centrifuging blood in a short phase of 600 g, discarding the bottom that contains the most of the red blood cells centrifuging the supernatant, most plasma, in a second phase to 1000 g extracting plasma closest to the interface with red blood cells focus through a phase of ultrafiltration, with different pore by the factor or factors you want to isolate and cooling the resulting pellet, or freezing it at -20 deg C or -70 deg C on the basis of which involves for the same immediate use or use the medium to long term. ACTIVITY : Vulnerary Osteopathic Ophthalmological. No biological data given. MECHANISM OF ACTION : Cell Therapy.