Line immortalized human epithelial cells of the cornea.

摘要

ESTIRPE immortalized EPITHELIAL CELLS HUMAN THE CAPABLE CORNEA stratifying AND EXPRESSING (1) MARKERS METABOLIC SPECIFIC EPITHELIAL CELLS HUMAN nonimmortalized AS vimentin, cytokeratins, CONNECTIONS BETWEEN CELLS, CYTOCHROME P450, A glutathione S-transferase, CU / ZNSUPEROXIDO dismutase, glutathione peroxidase, catalase aldehyde reductase; (2) DIFFERENTIATION MARKERS Metabolic NO SPECIFIC Epithelial Cells Immortalized human corneal AS OF 64 kD cytokeratin, glutathione-S-transferases HGST5.8 and profile of cytokines and growth factors which encompasses compounds AL TNF, IL BE -1, IL-1 AL, IL-6, IL-8, GM CSF BE, IL-RA, TGF BE 1, BE 2 TGF, TGF AL, EGF, PDGF BE; (3) AND MARKERS SPECIFIC INFLAMMATORY REACTION, such as collagenase I, bradykinin receptor, histamine and PAF SYSTEM AND A SIGNAL TRANSDUCTION BY INFLAMMATORY phosphoinositide. METHOD TO IDENTIFY THE EFFECT mutagen, toxic or beneficial of an agent on the metabolism of corneal cells, in which (1) are reacted, cultured or brought into contact with a culture comprising a cell line according to the invention, an agent attributed PROPERTIES mutagenic, toxic or beneficial for the metabolism of the cells of the human cornea; AND (2) ARE DETERMINED OR measure the effects of said agent on said CELL LINE.