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Assay for detecting an analyte containing or labelled with a haem moiety
Assay for detecting an analyte containing or labelled with a haem moiety
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机译:检测含有血红素部分或标记有血红素部分的分析物的分析
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摘要
A method for detecting an analyte containing or labelled with a haem moiety within a sample, said method comprising: a) contacting said sample with a magnetic bead having immobilised thereon a specific binding partner for said analyte and allowing analyte to bind to said specific binding partner; b) separating the magnetic beads from the sample, and if necessary, labelling the immobilised analyte with a haem containing label; c) resuspending the beads and subjecting them to alkaline conditions sufficient to release haem moieties therefrom but not to extract inorganic iron from the beads; d) detecting released haem moieties using a luminol chemiluminescent assay procedure.
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