EXPRESSION VECTORS CONTAINING A TRUNCATED EPSTEIN BARR NUCLEAR ANTIGEN 1 LACKING THE GLY-GLY-ALA DOMAIN FOR ENHANCED TRANSIENT GENE EXPRESSION

摘要

This invention relates to the unexpected discovery that nucleotide coding sequences coding for a truncated Epstein Barr Nuclear Antigen 1 (EBNA1t) protein (lacking the Gly-Gly-Ala domain), when in cells of mammalian origin, are associated with improved growth and increased transient gene expression when compared with cells expressing a complete EBNA1 coding sequence. The expression of EBNA1t also appear to be more stable over time.