VITAL CELL-CONTROLLING APPARATUS AND VITAL CELL-CONTROLLING METHOD

摘要

It is intended to provide a vital cell-controlling apparatus whereby cell organelles in a fluorescent stained cell are irradiated with ultrashort light pulses in the near infrared region to induce an adequate dysfunction and a method thereof. The vital cell-controlling method is conducted by focus-irradiating a stained vital sample with a light having a wavelength shorter than or almost the same as visible lights, extracting the florescence thus emitted from an excited fluorescent substance, visualizing the location of a cell organelle in the vital cell by displaying the image depending on the extracted fluorescence and thus performing fluorescent monitoring. Based on the visualized image display, a target cell organelle is selected from among the cell organelles and then the target cell organelle is irradiated with ultrashort light pulses in the near infrared region. Further, the vital sample having been irradiated with the ultrashort light pulses is fluorescent monitored. Thus, the displayed images of the vital sample before and after the irradiation with the ultrashort light pulses are compared so as to determine appropriate energy conditions for the ultrashort light pulses corresponding to the dysfunction of the desired cell organelle.