FABRICATION OF CARBOHYDRATE CHIPS BY IMMOBILIZING UNMODIFIED CARBOHYDRATES ON DERIVATIZED SOLID SURFACES, AND THEIR USES

摘要

The present invention relates to the fabrication of carbohydrate chips wherein free carbohydrates are immobilized on a modified solid surface. More specifically, it relates to a fabrication method for carbohydrate chips wherein unmodified carbohydrates irrespective of their size are site-specifically and covalently attached to the solid surface derivatized by hydrazide or aminooxy groups. According to the present method, unmodified carbohydrates are efficiently immobilized on the solid surface regardless of their size, and carbohydrate chips are easily fabricated because the method does not require modified carbohydrates. Furthermore, the invention relates to the use of the fabricated carbohydrate chips for the rapid analysis of carbohydrate-protein interactions and the diagnosis of carbohydrate-based diseases.