ISOLATION OF THE BIOSYNTHESIS GENES FOR PSEUDO-OLIGOSACCHARIDES FROM STREPTOMYCES GLAUCESCENS GLA.O AND THEIR USE

摘要

The invention relates to: a recombinant DNA molecule containing genes for the biosynthesis of acarboses and homologous pseudo-oligosaccharides; oligonucleotide primers for the PCR amplification of the molecule; proteins obtainable by expression of the genes localised on the molecule; vectors and host cells containing said DNA molecule; proteins coded by the DNA molecule; proteins expressed by said vectors in said host cells; processes for producing acarboses by the infiltration and/or exclusion of the characteristic genes in corresponding host organisms; processes for completing the gene cluster of biosynthesis genes for acarboses; processes for isolating analogous gene clusters in other organisms than streptomyces glaucescens GLA.O; process for mutating promoters of endogenous acarbosis biosynthesis genes to increase the output of acarboses; use of streptomyces glaucescens GLA.O for the production of acarboses; and mutants of streptomyces glaucescens GLA.O optimised in relation to the acarbose yield.