A GENE ENCODING ANTIBACTERIAL PROTEIN OF FENNEROPENAEUS CHINENSIS AND ITS RECOMBINATION EXPRESSION METHOD AND USES

摘要

A gene encoding antibacterial protein of Fenneropenaeus chinensis, its recombination expression method and uses are disclosed. The gene has the base sequence of SEQ ID No. 1 and encodes the protein having the amino acid sequence shown in SEQ ID No. 2. A vector pCR®T7/NT TOPO®TA recombination and host bacterium BL21 (DE3) pLysS which can express the antibacterial protein in vitro using prokaryotic expression system are obtained. The recombination protein can be expressed in high efficiency by the host bacterium. On the basis of the characteristic of inclusion of several disulfide bonds in the recombination protein, oxidation-reduction system consisting of oxidative glutathion and reductive glutathion is used in the isolation, purification and renaturation of the expressed protein. Thus a recombination protein having antibiotic activity is acquired. The high efficiency expression and renaturation of the recombination protein can be achieved. In vitro verification of biological function of the recombination protein confirmed that it has strong inhibiting effect on gram-positive bacteria and has some inhibiting effect on gram-negative bacteria as well.