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METHOD FOR IMPROVEMENT IN TRANSFORMATION EFFICIENCY OR HOMOLOGOUS RECOMBINATION EFFICIENCY IN CELL BY USING DNA OLIGOMER AS CARRIER FOR GENE DELIVERY, AND CELL TRANSFORMED USING THE METHOD
METHOD FOR IMPROVEMENT IN TRANSFORMATION EFFICIENCY OR HOMOLOGOUS RECOMBINATION EFFICIENCY IN CELL BY USING DNA OLIGOMER AS CARRIER FOR GENE DELIVERY, AND CELL TRANSFORMED USING THE METHOD
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机译:以DNA寡聚体为载体的基因载体提高细胞转化效率或同型重组效率的方法,并通过该方法转化细胞
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摘要
The object is to develop a method and a composition which can improve the efficiency of homologous recombination between a modified nucleic acid and an intercellular nucleic acid in a cell (particularly, an animal cell or a plant cell); to prepare a transformed cell using the method; and to improve the efficiency of transformation of a host cell with an exogenous nucleic acid. The efficiency of homologous recombination can be improved by introducing a carrier nucleic acid that is homologous to a modified nucleic acid and/or an intracellular nucleic acid together with the modified nucleic acid into a host cell. The efficiency of transformation can be improved by introducing both an exogenous nucleic acid together with a carrier nucleic acid.
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