SCREENING METHOD AND IDENTIFICATION METHOD FOR SUBSTANCE CAPABLE OF INHIBITING DEGRANULATION REACTION OR PROSTAGLANDIN D2 PRODUCTION IN MAST CELL THROUGH Rec168, AND THERAPEUTIC AGENT FOR INFLAMMATORY DISEASE COMPRISING THE SUBSTANCE

摘要

The object is to provide: a method for screening a substance capable of regulating degranulation or inhibiting prostaglandin D2 production; and a method for identification of a substance capable of inhibiting the degranulation or prostaglandin D2 production in a mast cell, these methods being useful for developing a therapeutic agent for an allergic disease or an autoimmune disease based on a new mechanism through a G-protein conjugated receptor Rec168. Disclosed is a method for screening of a substance capable of inhibiting the signal transduction capability or a substance capable of regulating the degranulation or inhibiting the prostaglandin D2 production in a mast cell through Rec168. The method comprises the steps of: determining the signal transduction capability of a ligand having a Rec168-agonistic activity toward Rec168 in the presence or absence of a substance to be tested; and comparing a measurement value obtained in the presence of the substance with a measurement value obtained in the absence of the substance. Also disclosed is a method for identification of a substance capable of inhibiting the degranulation or prostaglandin D2 production in a mast cell.