A METHOD OF PRODUCING NUCLEAR TRANSFER EMBRYONIC STEM CELL BY SOMATIC CELL NUCLEAR TRANSPLANTATION TECHNIQUE

摘要

The method of producing nuclear transfer embryonic stem cell by somatic cell nuclear transplantation technique is a method of producing cell by somatic cell nuclear transplantation technique, which comprises transferring the nucleus of nerve cell of adult animal into the cytoplast of enucleated embryonic stem cell of animal embryo using micromanipulator, reconstituting nuclear transfer embryonic stem cell having novel genotype, and culturing nuclear transfer embryonic stem cell to make it dissociate and proliferate up to 2⁸, which embryonic stem cell and nerve cell are allogenic, and the resultant cell is nuclear transfer embryonic stem cell having donor nuclear cell-nerve cell genotype. The present invention resolves not only the ethical problem of the use of embryonic stem cell in medicine, but also the problem of immunological rejection, which occurs in cell transplantation. It still resolves the cell cycle synchronization of donor nuclear cell and recipient nuclear cell and the source problem of recipient nuclear cell in somatic cell nuclear transplantation, so it would doubtless accelerate the development of human embryonic stem cell including the development of cell engineering and tissue engineering from this.