KINASE AND PHOSPHATASE ASSAYS CONDUCTED BY ELEMENTAL ANALYSIS

摘要

Assay methods and kits for kinase and phosphatase enzymes involved in post-translational modifications of proteins are provided. The methods employ elemental analysis, including inductively coupled plasma mass spectrometry (ICP-MS), in conjunction with metal ion coordination complexes such as titanium dioxide or element-tagged antibodies, both of which may have affinity for phosphate groups. The methods allow for convenient and accurate assays for enzymes involved in post-translational modifications of substrates.