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Method of manufacturing a hapten between doxycycline and bovine serum albumin and application for detecting doxycycline in humans or animals
Method of manufacturing a hapten between doxycycline and bovine serum albumin and application for detecting doxycycline in humans or animals
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机译:强力霉素和牛血清白蛋白之间的半抗原的制备方法及其在人或动物中检测强力霉素的应用
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摘要
Method for preparing a hapten (I) by Mannich reaction between doxycycline (II) and bovine serum albumen (BSA) comprises (a) dissolving (II) and BSA in presence of formaldehyde at pH 4-5; (b) incubation of the mixture at 25-50[deg]C for at least 12 hours and (c) purification and isolation of the (II)-BSA conjugate formed by passage through a desalting column. ACTIVITY : Antimalarial. MECHANISM OF ACTION : None given.
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