A ISOLATION METHOD TRITERPENOID FROM RUMEX JAPONICUS AND A TRITERPENOID

摘要

A method of isolating a triterpenoid compound from Rumex japonicus Houtt. and a triterpenoid compound are provided to restrain the production of the advanced glycation end products(AGEs) and be used for treating and preventing diabetes complications. The triterpenoid compound separated from Rumex japonicus Houtt. is characterized by 2 alpha, 3 alpha, 19 alpha-trihydroxy-24-norurc-4(23),12-dien-28-oicacid. The method of isolating a triterpenoid compound comprises the steps of: (a) obtaining crude extract of Rumex japonicus Houtt. by reduced pressure concentration, after extracting the Rumex japonicus Houtt. using 20-80% alcohol; (b) suspending the crude extract in distilled water, and obtaining normal hexane soluble fraction by adding normal hexane and separating normal hexane layer; (c) obtaining ethylacetate soluble fraction by adding ethylacetate in layer remaining after separating normal hexane layer and separating the ethylacetate layer; (d) obtaining 12 number of fractions by performing silica gel chromatography using concentration gradient of chloroform:methanol mixing solvent; (e) obtaining 10 number of fractions by subjecting no.5 fraction among 12 number of fractions to silica gel chromatography using concentration gradient of normal hexane:ethylacetate:methanol mixing solvent; (f) obtaining 2 alpha, 3 alpha, 19 alpha-trihydroxy-24-norurc-4(23),12-dien-28-oicacid by subjecting no.3 fraction among fractions of the step(e) to reverse silica gel chromatography using methanol:water mixing solvent.