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Control of plant viruses with its extract of endemic strain Serratia spp. Gsm01

机译:用地方性沙雷氏菌的提取物控制植物病毒。 Gsm01

摘要

An endemic microorganism, Serratia spp. Gsm01, for controlling plant viruses, extract of its cultured medium, and a composition for controlling plant viruses containing the same extract are provided to effectively control plant viruses including cucumber mosaic virus(CMV), tobacco mosaic virus(TMV) and potato virus Y(PVY) without toxicity to human and environments. The endemic microorganism, Serratia spp. Gsm01(KCCM-10738P), for controlling plant viruses is provided, wherein Serratia spp. Gsm01(KCCM-10738P) is isolated from the soil where ginseng is raised. The composition for controlling plant viruses comprises Serratia spp. Gsm01(KCCM-10738P) or its cultured medium as an effective ingredient. The cultured medium extract of Serratia spp. Gsm01(KCCM-10738P) is prepared by (1) culturing Serratia spp. Gsm01(KCCM-10738P) in Muller Hinton medium at 25-30 deg. C for 12-72 hours, (2) heating the cultured medium at 95-100 deg. C for 10-30 minutes, and extracting it with water, and (3) filtering the extract with ultracentrifugation to separate substances having molecular weight of 100,000 or more, and subjecting the separated substances to gel filtration chromatography.
机译:一种地方微生物,沙雷氏菌。提供用于控制植物病毒的Gsm01,其培养基的提取物以及包含该提取物的用于控制植物病毒的组合物,以有效地控制植物病毒,包括黄瓜花叶病毒(CMV),烟草花叶病毒(TMV)和马铃薯病毒Y( PVY)对人类和环境无毒。地方微生物,沙雷氏菌。提供了用于控制植物病毒的Gsm01(KCCM-10738P),其中沙雷氏菌属。 Gsm01(KCCM-10738P)从人参生长的土壤中分离出来。用于控制植物病毒的组合物包含沙雷氏菌。 Gsm01(KCCM-10738P)或其培养基为有效成分。沙雷氏菌的培养基提取物。 Gsm01(KCCM-10738P)通过(1)培养沙雷氏菌(Serratia spp。)在25-30度的Muller Hinton培养基中的Gsm01(KCCM-10738P)。保持温度12-72小时,(2)在95-100℃下加热培养基。在50℃下洗涤10-30分钟,并用水萃取,(3)通过超速离心过滤萃取物以分离出分子量为100,000或更大的物质,然后对分离出的物质进行凝胶过滤层析。

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