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Method for Single-Molecule Detection in Cell Using a Combination of Differential Interference Contrast Microscopy and Total Reflection Fluorescencee Microscopy and the Apparatus therefor
Method for Single-Molecule Detection in Cell Using a Combination of Differential Interference Contrast Microscopy and Total Reflection Fluorescencee Microscopy and the Apparatus therefor
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机译:差动干涉显微镜和全反射荧光显微镜相结合的细胞单分子检测方法及其装置
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摘要
invention is transmitted by differential interference microscopy in living cells of the exact image of a measurement, a sample of very soon, without movement, and applying one or a total internal reflection fluorescence microscopy technique using two or more light sources of different wavelengths. The present invention using the prism to the left and right and up and down on both sides without any movement of the sample surface is well transmitted through the light, the total reflection of the total reflection prism form to induce the fluorescence and transmission light microscopy techniques differential interference microscope described using a combined method The two methods are applied together the living cells in the sample as well, one or two or more correct distribution of one or two or more single biomolecules caused by the light sources of different wavelengths from a single biomolecule in the cells of the measurements and the operation and properties of a single biomolecule can be analyzed in real time.
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