首页> 外国专利> A KIT FOR DISCRIMINATING GENETICAL IDENTIFICATION AMONG CERVUS SPECIES BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD

A KIT FOR DISCRIMINATING GENETICAL IDENTIFICATION AMONG CERVUS SPECIES BY RESTRICTION FRAGMENT LENGTH POLYMORPHISM METHOD

机译:限制性片段长度多态性方法识别鹿种的遗传识别的工具包

摘要

A kit for discriminating genetic identification among Cervus species through restriction fragment length polymorphism(RFLP) method is provided to supply accurate Cervus sample and be utilized for managing the quality of the Cervus. The method for discriminating genetic identification among Cervus species comprises the steps of: (a) extracting DNA from a Cervus sample; (b) preparing a reaction solution including the extracted DNA, a sense or an anti-sense primer; (c) amplifying the reaction solution; and (d) performing a RFLP on the amplified DNA to genotype it. The discrimination kit analyzes the genetic variation among Cervus species through the RFLP method including a PCR reagent containing a DNA extract reagent, a sense primer or an anti-sense primer and a restriction enzyme.
机译:提供了一种通过限制性片段长度多态性(RFLP)方法对子宫颈物种进行遗传鉴定的试剂盒,以提供准确的子宫颈样品,并用于管理子宫颈的质量。区分宫颈物种的遗传鉴定的方法包括以下步骤:(a)从宫颈样品中提取DNA; (b)制备包括提取的DNA,有义或反义引物的反应溶液; (c)放大反应溶液; (d)对扩增的DNA进行RFLP以对其进行基因分型。鉴别试剂盒通过RFLP方法分析了宫颈物种之间的遗传变异,该方法包括含有DNA提取试剂,有义引物或反义引物和限制酶的PCR试剂。

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