CULTIVATION METHOD OF FOMITOPSIS PINICOLA MYCELIUM USING CITRUS PEEL EXTRACT OR GREEN TEA EXTRACT AND FOMITOPSIS PINICOLA MYCELIUM THEREOF

摘要

A method of cultivating Fomitopsis pinicola mycelium by culturing a strain of Fomitopsis pinicola in a medium containing citrus peel or green tea extract is provided, which increases the mycelium yield and content of functional material containing free amino acid or polysaccharides as compared with using general synthetic media. First, a strain of Fomitopsis pinicola prepared by isolating Fomitopsis pinicola mycelium from Fomitopsis pinicola fruit body. Second, ground citrus peel or green tea leaf is extracted with hot water and filtered to produce citrus peel or green tea extract. Finally, the strain of Fomitopsis pinicola is inoculated into a medium and cultured at a pH of 4.0 to 6.0, 150 to 200rpm and 25 to 30deg.C. The medium contains 0.5 to 3.0%(v/v) of citrus peel or green tea extract, 3.0 to 7.0%(v/v) of glucose, 0.5 to 1.5%(w/v) of yeast extract, 0.1 to 1.5%(w/v) of peptone, 0.1 to 0.7%(w/v) of malt extract and 86.3 to 95.8%(v/v) of purified water.