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A NEW PRIMER SET AND A SELECTIVE METHOD OF HANWOO USING SAID PRIMER SET

机译:一种新的Primer Set和使用Said Primer Set的韩屋选择方法

摘要

A primer set and a selective method of Hanwoo by using the same primer set are provided to evaluate genetic ability of Hanwoo at any growth period precisely and conveniently by analyzing the presence of DNA marker related to economic traits of Hanwoo. A polynucleotide molecule specific to the economic traits of Hanwoo comprises the nucleotide sequence of SEQ ID NO:2 in the 5' terminal region and the nucleotide sequence of SEQ ID NO:3 in the 3' terminal region, contains a repetitive nucleotide sequence of (CA)15-23, and has total base number of 138-164bp. The primer set for detecting the gene specific to the economic traits of Hanwoo comprises the nucleotide sequences of SEQ ID NO:2 and SEQ ID NO:3, or complementary nucleotide sequences thereof. The Hanwoo having excellent economic traits is selected by performing PCR with the DNA obtained from the blood or cell of Hanwoo as a template using the primer set, and verifying whether the PCR product has the total base number of 138-164bp or not, wherein the economic traits of Hanwoo include daily weight gain, marbling score, backfat thickness and loin muscle area.
机译:通过分析与汉宇经济性状相关的DNA标记物的存在,提供了一种引物组和使用相同引物对汉宇的选择性方法,以在任何生长期准确,方便地评估汉宇的遗传能力。对Hanwoo经济性状特异的多核苷酸分子在5'末端区域包含SEQ ID NO:2的核苷酸序列,在3'末端区域包含SEQ ID NO:3的核苷酸序列,包含( CA)15-23,总碱基数为138-164bp。用于检测对韩宇的经济性状特异的基因的引物组包含SEQ ID NO:2和SEQ ID NO:3的核苷酸序列或其互补核苷酸序列。通过使用引物对从汉宇血液或细胞中获得的DNA作为模板进行PCR,并验证PCR产物的总碱基数是否为138-164bp,从而选择具有良好经济性的汉宇。 Hanwoo的经济特征包括每日体重增加,大理石花纹得分,后脂肪厚度和腰部肌肉面积。

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