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PRIMER FOR IDENTIFICATION OF SEX IN ASPARAGUS AND METHOD OF IDENTIFICATION OF SEX IN ASPARAGUS
PRIMER FOR IDENTIFICATION OF SEX IN ASPARAGUS AND METHOD OF IDENTIFICATION OF SEX IN ASPARAGUS
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机译:天蝎座中性别鉴定的基础和天蝎座中性别鉴定的方法
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摘要
A method for identifying the sex of Asparagus officinalis L. is provided to be able to reliably discriminate the sex of the Asparagus officinalis L. at the seedling stage, thereby being usefully used for increasing the number of the Asparagus officinalis L. stably and developing new breeding. The primer for identifying sex of Asparagus officinalis L. comprises an oligonucleotide consisting of SEQ ID : NO.1 and an oligonucleotide consisting of SEQ ID : NO.2. The method for identifying the sex of Asparagus officinalis L. comprises the steps of: (a) amplifying DNA extracted from the Asparagus officinalis L. through PCR using the primer; and (b) electrophoresing the amplified product to identify an Asparagus officinalis L. specific band having a size of 395bp consisting of SEQ ID : NO.3.
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机译:本发明提供了一种鉴别芦笋性别的方法,其能够在苗期可靠地鉴别芦笋的性别,从而有效地用于稳定地增加芦笋的数量并开发新的芦笋。配种。用于鉴别芦笋性别的引物包括由SEQ ID:NO.1组成的寡核苷酸和由SEQ ID:NO.2组成的寡核苷酸。鉴别芦笋性别的方法包括以下步骤:(a)使用引物通过PCR扩增从芦笋中提取的DNA; (b)对扩增产物进行电泳,以鉴定具有SEQ ID NO:3的大小为395bp的芦笋特异条带。
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