VECTOR SYSTEM FOR EXPRESSING SOLUBLE PROTEIN WITH HIGH EFFICIENCY

摘要

The invention object relates to a construction method of expression vector and the best expression system using the same to achieve the expression and availability of the proteins at the maximum, more specifically,, a) a promoter, b) histidine attached to enable the target protein expression the Taq binding, fusion to increase the target protein aqueous partner, c) TEV (Tobacco Etch Virus) protease recognition sequences, d) an amino acid linker, consisting of 2 to 10 pieces, and e) contains a multiple cloning seat (multicloning site) which provides an expression vector. The present invention can determine whether the availability increase of the target protein through various fusion partners coupled to quickly, you can select the recombinants looks the best expression, and availability, the fusion partner and a high cutting efficiency and precision between the target protein It provides a terminal. ; Target protein, the fusion partner, expression availability, multiple cloning spot