Methods for Isolating Corneal Stem Cells and Reconstructing Cornea

摘要

The present invention comprising the steps of: (a) by treating a di-SPA azepin each vague (limbus) separating the epithelial sheets; (B) to obtain a vague each epithelial cells of a single cells by treatment with trypsin-EDTA mixture and in the separated epidermal sheet; And (c) on each of the loosely epithelial cells of the culture by separation of the corneal stem cells, comprising the step of obtaining the corneal stem cells, methods, compositions for corneal stem cell separation method reconstructed corneal and artificial cornea. According to the invention, the prior art methods in particular extreme plant culture and in one-to successfully reconstruct the cornea may remove the corneal stem cells in 7-20 times the efficiency compared to the method step, by using the thus-separated corneal stem cells can do. ; Corneal, each vague, stem cells, and separate, three-dimensional culture, di azepin Spa, trypsin, EDTA