MUTANTS HAVING HIGHER PRODUCTION OF COENZYME Q10 AND PRODUCTION METHOD OF COENZYME Q10

摘要

Mutants having higher production of coenzyme Q10 and a production method of coenzyme Q10 by using the same mutants are provided to increase productivity of coenzyme Q10 and improve high viscosity of the finally fermented solution which is problem caused in former method by conferring tolerance against structure analogues of coenzyme Q10 and respiration inhibitors on the coenzyme Q10-producing microorganisms. The coenzyme Q10-producing mutant Agrobacterium tumefaciens AD 409(KCTC 10916BP) is produced by mutating a parent microorganism of Agrobacterium tumefaciens ATCC 4452 and has tolerance against dihydrocapsaicin and potassium cyanide. The coenzyme Q10 is produced by culturing Agrobacterium tumefaciens AD 409(KCTC 10916BP) in a medium containing 1-10% of molasses and 1-10% of glucose, and additionally containing 10-50 mg/l of thiamine, 10-50 mg/l of nicotinic acid and 0.01-0.05 mg/l of biotin.