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Elemicin Purified from Asarum sieboldii Miq. and Preparation Method thereof
Elemicin Purified from Asarum sieboldii Miq. and Preparation Method thereof
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机译:Elemicin从细辛细辛中提取。及其制备方法
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摘要
An herbicidal substance, elemicin, purified from Asarum sieboldii Miq. and a preparation method thereof are provided to serve a novel herbicide having different chemical structure compared with conventional herbicides, so that it will be a lead compound for development of new herbicides. The method for preparing the elemicin having herbicidal effects purified from Asarum sieboldii Miq. comprises the steps of: (a) extracting Asarum sieboldii Miq. with methanol and drying the extract; (b) dissolving the methanol extract of Asarum sieboldii Miq. in hexane to prepare a hexane fraction; (c) subjecting the hexane fraction to column chromatography using hexane-ethylacetate-methanol solution(5:1:0.1 v/v/v) to obtain HB fraction; (d) subjecting the HB fraction to column chromatography using hexane-ethylacetate-methanol solution(9:1:0.5 v/v/v) to obtain HBB fraction; (e) subjecting the HBB fraction to column chromatography using hexane-ethylacetate solution(5:5 v/v) to obtain HBBA fraction; and (f) subjecting the HBBA fraction to affinity column chromatography using methanol-acetonitrile solution(9:1 v/v) to obtain HBBAA fraction.
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机译:从细辛细辛(Asarum sieboldii Miq)纯化的除草剂elemicin。本发明提供了一种其化学结构与常规除草剂不同的新型除草剂的制备方法,从而成为开发新型除草剂的先导化合物。从细辛细辛中提取的具有除草作用的elemicin的制备方法。包括以下步骤:(a)提取细辛细辛。用甲醇干燥提取物; (b)将细辛细辛的甲醇提取物溶解。在己烷中制备己烷馏分; (c)用己烷-乙酸乙酯-甲醇溶液(5∶1∶0.1v / v / v)对己烷馏分进行柱层析,得到HB馏分; (d)用己烷-乙酸乙酯-甲醇溶液(9:1:0.5 v / v / v)对HB馏分进行柱色谱分离,得到HBB馏分; (e)用己烷-乙酸乙酯溶液(5:5 v / v)对HBB馏分进行柱层析,得到HBBA馏分; (f)用甲醇-乙腈溶液(9∶1 v / v)对HBBA馏分进行亲和柱层析,得到HBBAA馏分。
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