Reagents for determination of the DNA of microbes PARODONTOPATOGENNYH PREVOTELLA INTERMEDIA SENSU STRICTO, BACTEROIDES FORSYTHUS, TREPONEMA DENTICOLA, ACTINOBACILLUS ACTINOMYCETEMCOMITANS, PORPHYROMONAS GINGIVALIS WITH MULTIPLEX POLYMERASE CHAIN ​​REACTION

摘要

1.a set of reagents u0434u043bu00a0 u043eu043fu0440u0435u0434u0435u043bu0435u043du0438u00a0 dna u043fu0430u0440u043eu0434u043eu043du0442u043eu043fu0430u0442u043eu0433u0435u043du043du044bu0445 germs Prevotella intermedia sensu stricto, Bacteroides forsythus, Treponema denticola, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis using multi u043fu043bu0435u043au0441u043du043eu0439 with an r.l.f.t...., u0441u043eu0441u0442u043eu00a0u0449u0438u0439 of reagent kit u0434u043bu00a0 sample preparation, kit u0434u043bu00a0 u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u0438 comprising the reaction mixture u0441u043eu0434u0435u0440u0436u0430u0449 gear buffer.4 u043fu0440u00a0u043cu044bu0435 and 2 reverse u043fu0440u0430u0439u043cu0435u0440u044b, u0434u0435u0437u043eu043au0441u0438u043du0443u043au043bu0435u043eu0437u0438u0434u0442u0440u0438u0444u043eu0441u0444u0430u0442u044b, paint u0434u043bu00a0 electrophoresis, dna u043fu043eu043bu0438u043cu0435u0440u0430u0437u0443, positive and negative control dna samples and computer u043bu0435u043au0442u0430 u0434u043bu00a0 analysis of pcr products, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 the reagent kit u0434u043bu00a0 u0430u043cu043fu043bu0438u0444u0438u043au0430u0446u0438u0438 added u043fu0440u00a0u043cu043eu0439 u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u043du044bu0439 primer.specific u0434u043bu00a0 Prevotella intermedia sensu stricto genome fragment with a sequence of 5 '- GCA tct GAC GTG GAC saha - 3', u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0 0.15 microns, two u043eu043bu0438u0433u043eu043du0443u043au043bu0435u043eu0442u0438u0434u043du044bu0445 primer, u0444u043bu0430u043du043au0438u0440u0443u044eu0449u0438u0435 site specific genomic dna u0434u043bu00a0 Treponema denticola with u043fu043eu0441u043bu0435u0434u043eu0432u0430u0442u0435u043bu044cu043du043eu0441u0442u00a0u043cu0438 5 '- GAA tcg 100 gta pbx GCA cat CAG - 3' and 5 '- u0442u0442u0441 aaa cat TCC AGC gtc u0442u0442u0441 attus - 3', with each of the n u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0 it is 0.30 microns, and u043au043eu043du0446u0435u043du0442u0440u0430u0446u0438u00a0 each u0434u0435u0437u043eu043au0441u0438u043du0443u043au043bu0435u043eu0437u0438u0434u0442u0440u0438u0444u043eu0441u0444u0430u0442u0430 u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 250 microns, a positive control dna sample consists of plasmid dna u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu0439 pPIN with clo u043du0438u0440u043eu0432u0430u043du043du044bu043c fragment size of 1000 u043f.u043d. genome Prevotella intermedia sensu stricto, plasmid dna u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu0439 pBFO with u043au043bu043eu043du0438u0440u043eu0432u0430u043du043du044bu043c fragment size of 745 u043f.u043d.Bacteroides forsythus genome, dna u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu0439 plasmid pTD with u043au043bu043eu043du0438u0440u043eu0432u0430u043du043du044bu043c fragment size of 512 u043f.u043d. genome Treponema denticola, u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu0439 plasmid dna fragment size of raas with u043au043bu043eu043du0438u0440u043eu0432u0430u043du043du044bu043c 360 u043f.u043d. Actinobacillus actinomycetemcomitans genome, dna u0440u0435u043au043eu043cu0431u0438u043du0430u043du0442u043du043eu0439 plasmid pPGI with u043au043bu043eu043du0438u0440u043eu0432u0430u043du043du044bu043c fragment size of 197 u043f.u043d. genome Porphyromonas gingivalis.;2. set to 1, u043eu0442u043bu0438u0447u0430u044eu0449u0438u0439u0441u00a0 so that as the buffer using tris - (hydroxymethyl) - u0430u043cu0438u043du043eu043cu0435u0442u0430u043d, 670 mm ammonium sulphite, 170 mm magnesium chloride, 20 mm, ph 8.8.