Permanent Link ovary cells DOMESTIC GOAT CAPRA HIRCUS L. YADK-04 for the reproduction of animal viruses

摘要

u043bu0438u043du0438u00a0 cells of animal u044fu0434u043a - 04, u0434u0435u043fu043eu043du0438u0440u043eu0432u0430u043du043du0430u00a0 6 u043eu043au0442u00a0u0431u0440u00a0 2004. in the collection u043fu0435u0440u0435u0432u0438u0432u0430u0435u043cu044bu0445 somatic cells u0441u0435u043bu044cu0441u043au043eu0445u043eu0437u00a0u0439u0441u0442u0432u0435u043du043du044bu0445 and fishing all russian research institute of experimental animals in veterinary medicine (u0432u0441u043au043a u0441u0445u0436 u0440u0430u0441u0445u043d) 63, u0434u043bu00a0 reproduction of viruses of animals, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0430u00a0u0441u00a0 what she;u0432u043bu00a0u0435u0442u0441u00a0 line cells of goats and Capra hircus.;the cells obtained from the u043fu043eu0441u0442u043eu00a0u043du043du043eu0439 u0433u043eu043du0430u0434 goats cg - 91 through adaptation to the growth environment of the salt solution in the u0445u0435u043du043au0441u0430 monolayer of planting density of less than 5 0 the tc / ml;;is mixed to u043au0430u0440u0438u043eu043bu043eu0433u0438u0447u0435u0441u043au0438u043c marks u043fu043eu043fu0443u043bu00a0u0446u0438u044e cells, mainly u0430u043du0435u0443u043fu043bu043eu0438u0434u043du044bu0445, with u0434u043eu043bu00a0 cells with 57 - 58 chromosomes u0441u043eu0441u0442u0430u0432u043bu00a0u0435u0442 20 - 22%, with 54 - 61 u0445u0440u043eu043cu043eu0441u043e mami 63 - 67%, with 115 123 chromosomes 7 - 9%;;the cultivation in the monolayer of cyclical manner provides a replica of the virus u00a0u0449u0443u0440u0430 type and u0442u0438u0442u0440u043eu043c 6.0 - 6.5 lg u0442u0446u043450 / ml, the virus u00a0u0449u0443u0440u0430 type on the u0442u0438u0442u0440u043eu043c 5.0 - 5.5 lg u0442u0446u043450 / m l u00a0u0449u0443u0440u0430 u0430u0437u0438u00a0 virus type 1 u0442u0438u0442u0440u043eu043c 5.25 to 5.5 u0442u0446u043450 lg / ml, the virus disease u0430u0443u0435u0441u043au0438 with u0442u0438u0442u0440u043eu043c 8.0 8.5 lg u0442u0446u043450 / ml, the smallpox virus of sheep with u0442u0438u0442u0440u043eu043c 5.75 - 6.25 lg u0442u0446u043450 / ml, with classical plague virus vineuil, indre with u0442u0438u0442u0440u043eu043c 5.5 - 6.5 lg u0442u0446u043450 / mlu043fu043du0435u0432u043cu043eu0432u0438u0440u0443u0441u0430 birds with u0442u0438u0442u0440u043eu043c 5.0 - 5.5 u0442u0446u043450 / ml.