BACTERIUM Escherichia coli PRODUCING HETEROLOGOUS POLYPEPTIDE AND METHOD FOR PRODUCING POLYPEPTIDE

摘要

FIELD: biotechnology, microbiology, genetic engineering.;SUBSTANCE: invention relates to microorganism E. coli showing deficiency by chromosomal genes degP and prc that encode proteases DegP and Prc, respectively, and comprising mutant gene spr containing the E. coli suppressor sequence prc with the point mutation at position 148 that results to substitution of tryptophan for arginine at position 148. The heterologous polypeptide is prepared by culturing this microorganism. Invention provides preparing heterologous polypeptides showing the high degree of effectiveness.;EFFECT: valuable biological properties of microorganism.;23 cl, 13 dwg, 6 tbl, 2 ex