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DYES BASED ON DIPYRROMETHENE BORON DIFLUORIDE WITH BIPHOTONIC ABSORPTION AND THEIR USE
DYES BASED ON DIPYRROMETHENE BORON DIFLUORIDE WITH BIPHOTONIC ABSORPTION AND THEIR USE
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机译:基于双亚甲基二硼化硼的生物吸附性染料及其用途
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摘要
FIELD: physical analytical methods.;SUBSTANCE: invention relates to bioanalytical methods involving dye-labeled indicators. Bioanalytical method without separation is provided directed to measure analyte obtained from biological liquid or suspension, wherein are used: analyte microparticles as first biospecific reagent; and second biospecific reagent labeled with biphotonic fluorescent dye based on dipyrromethene boron difluoride containing at least one water solubility imparting group selected from ammonium salt and sulfonic or carboxylic acid alkali metal salt and at least one chemically active group selected from carboxylic acid, reactive carboxylic acid ester, carboxylic acid anhydride, maleimide, amine and isothiocyanate. In the method of invention, laser is focused onto reaction suspension and biphotonically excited fluorescence from individual microparticles (randomly flowing or oriented by pressure provided by emission of exciting laser through focal volume of laser beam).;EFFECT: increased efficiency of bioanalyses.;5 cl, 5 dwg, 5 tbl, 25 ex
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机译:技术领域本发明涉及涉及染料标记指示剂的生物分析方法。提供了不分离的生物分析方法,其用于测量从生物液体或悬浮液获得的分析物,其中使用:作为第一生物特异性试剂的分析物微粒;第二生物特异性试剂,其被基于二吡咯亚甲基二氟化硼的双光子荧光染料标记,该双光子荧光染料包含至少一种选自铵盐和磺酸或羧酸碱金属盐的水溶性赋予基团和至少一种选自羧酸,反应性羧酸酯的化学活性基团,羧酸酐,马来酰亚胺,胺和异硫氰酸酯。在本发明的方法中,将激光聚焦到反应悬浮液和来自单个微粒的双光子激发的荧光上(随机流动或通过通过激光束的聚焦体积发射激发激光而提供的压力来定向);效果:提高了生物分析的效率。5 cl,5 dwg,5 tbl,25 ex
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