TEST immunoassay for the semiquantitative determination of allergen-specific IgE and IgG-antibodies for the diagnosis of mycosis (IFTS-MICS-IgE-IgG-AT)

摘要

Test - immunoassay for the semiquantitative determination of allergen specific IgE and IgG - antibodies for diagnosis of fungal infections (IFTS-myco-IgE-IgG-AT) The utility model is used in the medical diagnostic laboratory. The task performed by the proposed test -system IFTS-myco-IgE-IgG-AT, developed by solid phase sandwich method in a 96-well plate stripirovannom using monoclonal antibodies comprising the peroxidase conjugates, of anti-mAb-IgE and MKAT- anti-IgG. The method is based on a combination of immunological reactions in the first stage between fungal allergens immobilized on a polystyrene microtiter plate, and specific to these allergens IgE - and IgG - the antibodies from the serum sample to form a solid phase immunological complexes AG _1-AT. In the second stage between complexes of _AH-1 and AT-conjugates MAbs and anti-IgE mAb-anti-IgG to form an immunological complex AT-AT ₁ -AT _2, labeled peroxidase enzyme. Subsequent enzymatic color reaction occurs between the peroxidase and the substrate. cleavage reaction products are converted chromogen molecules - TMB into a colored derivative whose amount is proportional to enzyme activity. The degree of staining is considered photometrically at 450 nm. Contents of allergen-IgE-IgG-antibodies in the samples is expressed in grades (1-4) corresponding to low, medium, high and moderately high concentrations of IgE and IgG. The utility model is a kit, which includes a kit comprising polystyrene plates with immobilized allergens reference - serum wash solution chromogen substrate, Stop - reagent and instructions for use that are packaged in one box.