alkaline protease variants and detergents and cleaning agents containing these alkaline protease variants

摘要

Described herein are novel alkaline protease variants derived from subtilisin. These variants have, with respect to the amino acid sequence of Bacillus lentus subtilisin, variations at amino acid positions 199 and 211, and at least one modification that contributes to the stabilization of the molecule, the modification preferably being variations at amino acid positions 3 and/or 4. Preferably, the variant is B. lentus alkaline protease S3T/NV4I/V199I/L211G. Also described are detergents and cleaning agents comprising the novel alkaline protease variants. Methods of use employing the novel alkaline protease variants are also described.