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method for the qualitative and / or quantitative determination of genus, species, breed and / or geographical origin of biological materials

机译:定性和/或定量确定生物材料的属,种,品种和/或地理起源的方法

摘要

A method for determining genus, species, race and/or geographical origin of biological materials, by directly cleaving with enzymatic methods the fibrillary structures such as feathers, down, hair or horn to form a pool of cleaved peptides, without prior dissolution of structure proteins; and wherein the pool of cleaved peptides obtained from fibillary structure proteins is preferably subjected to MALDI-TOF mass spectroscopy without the application of separating and isolating techniques, and wherein the mass spectrograms obtained are adjusted using reference mass spectrograms and the suitable specific mass peaks are used to quantify elements such as feathers, down, scales horn and/or hair which are foreign to the species.
机译:一种通过直接用酶方法切割纤维结构(例如羽毛,羽绒,毛发或角状物)以形成裂解肽库的方法来确定生物材料的属,种,种族和/或地理起源的方法,而无需事先溶解结构蛋白;其中从原纤维结构蛋白获得的裂解肽库优选在不使用分离和分离技术的情况下进行MALDI-TOF质谱分析,并且其中获得的质谱图使用参考质谱图进行调整,并使用合适的比重峰来量化该物种不熟悉的元素,例如羽毛,羽绒,鳞角和/或头发。

著录项

  • 公开/公告号DE50210929D1

    专利类型

  • 公开/公告日2007-10-31

    原文格式PDF

  • 申请/专利权人 ALTMEYER WOLFGANG;

    申请/专利号DE20025010929T

  • 申请日2002-05-15

  • 分类号G01N27/62;G01N33/483;C12Q1/37;G01N1/28;G01N27/447;G01N30/06;G01N30/88;G01N33/48;G01N33/50;G01N33/68;

  • 国家 DE

  • 入库时间 2022-08-21 20:28:40

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